DNA by intercalating between the bases with

Page 52

{"fact":"A cat's jaw has only up and down motion; it does not have any lateral, side to side motion, like dogs and humans.","length":113}

{"type":"standard","title":"Propidium iodide","displaytitle":"Propidium iodide","namespace":{"id":0,"text":""},"wikibase_item":"Q424169","titles":{"canonical":"Propidium_iodide","normalized":"Propidium iodide","display":"Propidium iodide"},"pageid":2279750,"thumbnail":{"source":"https://upload.wikimedia.org/wikipedia/commons/thumb/7/7d/Propidium_iodide.svg/330px-Propidium_iodide.svg.png","width":320,"height":225},"originalimage":{"source":"https://upload.wikimedia.org/wikipedia/commons/thumb/7/7d/Propidium_iodide.svg/725px-Propidium_iodide.svg.png","width":725,"height":510},"lang":"en","dir":"ltr","revision":"1262764339","tid":"5a192436-b8f1-11ef-b0c2-4cea20a57816","timestamp":"2024-12-13T01:27:01Z","description":"Chemical compound","description_source":"local","content_urls":{"desktop":{"page":"https://en.wikipedia.org/wiki/Propidium_iodide","revisions":"https://en.wikipedia.org/wiki/Propidium_iodide?action=history","edit":"https://en.wikipedia.org/wiki/Propidium_iodide?action=edit","talk":"https://en.wikipedia.org/wiki/Talk:Propidium_iodide"},"mobile":{"page":"https://en.m.wikipedia.org/wiki/Propidium_iodide","revisions":"https://en.m.wikipedia.org/wiki/Special:History/Propidium_iodide","edit":"https://en.m.wikipedia.org/wiki/Propidium_iodide?action=edit","talk":"https://en.m.wikipedia.org/wiki/Talk:Propidium_iodide"}},"extract":"Propidium iodide is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane integrity. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining. PI is widely used in fluorescence staining and visualization of the plant cell wall.","extract_html":"

Propidium iodide is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane integrity. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining. PI is widely used in fluorescence staining and visualization of the plant cell wall.

"}

{"fact":"The average cat food meal is the equivalent to about five mice.","length":63}

{"fact":"Normal body temperature for a cat is 102 degrees F.","length":51}

{"slip": { "id": 17, "advice": "Sometimes it's best to ignore other people's advice."}}

{"slip": { "id": 177, "advice": "Everyone has their down days. Don't take it out on innocent bystanders."}}

{"type":"standard","title":"Westhumble","displaytitle":"Westhumble","namespace":{"id":0,"text":""},"wikibase_item":"Q7988910","titles":{"canonical":"Westhumble","normalized":"Westhumble","display":"Westhumble"},"pageid":3538742,"thumbnail":{"source":"https://upload.wikimedia.org/wikipedia/commons/thumb/b/bf/Arch_Leading_to_%27Camilla%27_-_geograph.org.uk_-_398545.jpg/330px-Arch_Leading_to_%27Camilla%27_-_geograph.org.uk_-_398545.jpg","width":320,"height":240},"originalimage":{"source":"https://upload.wikimedia.org/wikipedia/commons/b/bf/Arch_Leading_to_%27Camilla%27_-_geograph.org.uk_-_398545.jpg","width":2848,"height":2136},"lang":"en","dir":"ltr","revision":"1285889976","tid":"f7aa7df3-1ab6-11f0-9637-72a08df887f3","timestamp":"2025-04-16T11:35:59Z","description":"Village in Surrey, England","description_source":"local","coordinates":{"lat":51.254,"lon":-0.333},"content_urls":{"desktop":{"page":"https://en.wikipedia.org/wiki/Westhumble","revisions":"https://en.wikipedia.org/wiki/Westhumble?action=history","edit":"https://en.wikipedia.org/wiki/Westhumble?action=edit","talk":"https://en.wikipedia.org/wiki/Talk:Westhumble"},"mobile":{"page":"https://en.m.wikipedia.org/wiki/Westhumble","revisions":"https://en.m.wikipedia.org/wiki/Special:History/Westhumble","edit":"https://en.m.wikipedia.org/wiki/Westhumble?action=edit","talk":"https://en.m.wikipedia.org/wiki/Talk:Westhumble"}},"extract":"Westhumble is a village in south east England, approximately 2 km (1.2 mi) north of Dorking, Surrey. The village is not part of a civil parish, however the majority of the settlement is in the ecclesiastical Parish of Mickleham.","extract_html":"

Westhumble is a village in south east England, approximately 2 km (1.2 mi) north of Dorking, Surrey. The village is not part of a civil parish, however the majority of the settlement is in the ecclesiastical Parish of Mickleham.

"}

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